Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19795] to CRISPR-Cas9 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Staphylococcus aureus

Overview

  • Product name

    Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free
    See all CRISPR-Cas9 primary antibodies

  • Description

    Rabbit monoclonal [EPR19795] to CRISPR-Cas9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Staphylococcus aureus

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T whole cell lysate transfected with Myc-His tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus) vector. ICC/IF: HEK-293T cells transfected with GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus) expression construct. Flow Cyt: HEK-293T cells transfected with GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus) expression construct. IP: HEK-293T cell lysate transfected with GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus) vector.

  • General notes

    Ab223153 is the carrier-free version of ab203943. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223153 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19795

  • Isotype

    IgG

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)
    Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CRISPR-Cas9 with ab203943 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) expression construct.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203943).

  • Flow Cytometry - Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)
    Flow Cytometry - Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector, labeling CRISPR-Cas9 with ab203943 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black). Goat anti rabbit IgG (Alexa Fluor® 647) at 1/2000 dilution was used as the secondary antibody.

    Note: The image is obtained by gating the GFP-tag positive population.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203943).

  • Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)
    Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)

    CRISPR-Cas9 was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector,  with ab203943 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203943 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector, 10 μg (Input).

    Lane 2: ab203943 IP in HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203943 in HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203943).

  • Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)
    Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free (ab223153)

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