Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)
![Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)](https://www.abcam.com/ps/products/251/ab251386/Images/ab251386-408223-anti-zwilch-antibody-epr18035-28-bsa-and-azide-free-western-blot.jpg "Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18035-28] to ZWILCH - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free
See all ZWILCH primary antibodies -
Description
Rabbit monoclonal [EPR18035-28] to ZWILCH - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251386 is the carrier-free version of ab202898. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251386 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR18035-28 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)All lanes : Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 4 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 15 secondsThis data was developed using ab202898, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)](https://www.abcam.com/ps/products/251/ab251386/Images/ab251386-405982-anti-zwilch-antibody-epr18035-28-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution + Mouse brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 3 minutesThis data was developed using ab202898, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)](https://www.abcam.com/ps/products/251/ab251386/Images/ab251386-403374-anti-zwilch-antibody-epr18035-28-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)All lanes : Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cells) cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 15 secondsThis data was developed using ab202898, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)](https://www.abcam.com/ps/products/251/ab251386/Images/ab251386-1-anti-zwilch-antibody-epr18035-28-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)This data was developed using ab202898, the same antibody clone in a different buffer formulation.ZWILCH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202898 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202898 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab202898 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202898 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds. -
![Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)](https://www.abcam.com/ps/products/251/ab251386/Images/ab251386-5-benefits-of-recombinant-antibodies.png)
Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (ab251386)