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Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)

Key features and details

  • Mouse monoclonal [TFF1/2133] to Estrogen Inducible Protein pS2 - BSA and Azide free
  • Suitable for: ICC/IF, Protein Array, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free
    See all Estrogen Inducible Protein pS2 primary antibodies

  • Description

    Mouse monoclonal [TFF1/2133] to Estrogen Inducible Protein pS2 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Protein Array, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human Estrogen Inducible Protein pS2 aa 1-84.
    Database link: P04155

  • Positive control

    • IHC-P: Human breast carcinoma tissue. ICC/IF: MCF7 cells. Flow cyt: MCF7 cels.

  • General notes

    ab268241 is the PBS only version of ab268118.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from Bioreactor concentrate

  • Clonality

    Monoclonal

  • Clone number

    TFF1/2133

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)
    Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)

    Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling Estrogen Inducible Protein pS2 with ab268118 at 2 μg/ml (Green). The secondary antibody is a Goat anti-mouse IgG CF488 conjugate. Membrane are labeled with phalloidin (Red).

    This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268118).

  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)
    Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)

     

    Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling Estrogen Inducible Protein pS2 with ab268118 at 2 μg/ml (Green). The secondary antibody is a Goat anti-mouse IgG CF488 conjugate. Membrane are labeled with phalloidin (Red).

    This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268118).

     

  • Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)
    Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)

     Flow cytometric analysis of PFA-fixed MCF7 cells cells labeling Estrogen Inducible Protein pS2 with ab268118 at 2 μg/106 cells (Blue) compared with an Isotype control (Red). The secondary antibody was a Goat anti-mouse IgG CF488 conjugate.

    This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268118).

  • Protein Array - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)
    Protein Array - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (ab268241)

    Protein Array containing more than 19,000 full-length human proteins using ab268118.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268118).

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