Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Inducible Protein pS2 using ab268118 at 2 μg/ml in immunohistochemical analysis.

Immunofluorescence staining of paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells stained for Estrogen Inducible Protein pS2 with ab268118 at 2 µg/million cells followed by a goat anti-Mouse IgG-CF488 (Green). Nucleus is labeled with Reddot (Red).

Flow Cytometric analysis of PFA-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Estrogen Inducible Protein pS2 using ab268118 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence staining of paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells stained for Estrogen Inducible Protein pS2 with ab268118 at 2 µg/million cells followed by goat anti-Mouse IgG-CF488 (Green). Membrane are labeled with phalloidin (Red).

Protein Array containing more than 19,000 full-length human proteins using ab268118.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.