Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12816] to FAM50A/XAP5
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FAM50A/XAP5 antibody [EPR12816]
    See all FAM50A/XAP5 primary antibodies

  • Description

    Rabbit monoclonal [EPR12816] to FAM50A/XAP5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, Hela 293, HepG2, C6, Raw264.7, PC-12, NIH-3T3 cell lysates, human colon tissue, Hela cells

  • General notes

     

     

     This product was previously labelled as FAM50A

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12816

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
    Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
    All lanes : Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa

  • Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
    Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
    All lanes : Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FAM50A/XAP5 with ab186410 at 1/500 and ready to use HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
    Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)

    Immunocytochemical analysis of Hela cells fixed in 4% paraformaldehyde labling FAM50A/XAP5 with ab186410 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution.

  • Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
    Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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