Anti-Ankrd26 antibody (ab47984)
Key features and details
- Rabbit polyclonal to Ankrd26
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ankrd26 antibody
See all Ankrd26 primary antibodies -
Description
Rabbit polyclonal to Ankrd26 -
Host species
Rabbit -
Specificity
This antibody is specific for mouse Ankrd26. -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment
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Immunogen
Synthetic peptide:
MKKIFGFRSKGPSPL
, corresponding to amino acids 1-15 of Mouse Ankrd26 -
Positive control
- MES cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ankrd26 antibody (ab47984)Lanes 2-10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution
Lane 1 : BLANK
Lane 2 : MES cell lysate - 80µg
Lane 3 : MES cell lysate - 40µg
Lane 4 : 293T-ANKRD26
transfected cell lysate - 20µg
Lane 5 : control 293T cell
lysate - 20µg
Lane 6 : BSA-ANKRD26 conjugate - 20ng
Lane 7 : BSA - 500ng
Lane 8 : BSA - 100ng
Lane 9 : BSA - 20ng
Lane 10 : Protein standards
Secondary
Lanes 2-10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution
Predicted band size: 196 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. An observed band of ~81kDa has been obtained due to a fragment of Ankrd26 being used instead of the full length version.
