Anti-PHF21B antibody [OTI3D1] (ab119249)
![Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-1.jpg "Anti-PHF21B antibody [OTI3D1] (ab119249)")
Key features and details
- Mouse monoclonal [OTI3D1] to PHF21B
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-PHF21B antibody [OTI3D1] -
Description
Mouse monoclonal [OTI3D1] to PHF21B -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human PHF21B. Produced in HEK293T cells (NP_612424.1).
Database link: Q96EK2 -
Positive control
- IHC-P: Human Breast, Kidney, Endometrium, Liver, Ovary Adenocarcinoma, Pancreas carcinoma, Lung carcinoma and Thyroid Carcinoma tissues. WB: HEK-293T cell lysate transfected with pCMV6-ENTRY PHF21B cDNA. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY PHF21B. Flow: HEK-293T cells transfected with pCMV6-ENTRY PHF21B overexpress plasmid.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3D1 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-PHF21B antibody [OTI3D1] (ab119249)All lanes : Anti-PHF21B antibody [OTI3D1] (ab119249) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY
control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY
PHF21B cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 57 kDa
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![Flow Cytometry - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-2.jpg)
Flow Cytometry - Anti-PHF21B antibody [OTI3D1] (ab119249)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PHF21B overexpress plasmid (Red) or empty vector control plasmid (Blue), stained using ab119249 at 1/100 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PHF21B antibody [OTI3D1] (ab119249)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY PHF21B, stained for PHF21B (green) using ab119249 at 1/100 dilution in ICC/IF.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human breast tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/ab119249-132168-anti-phf21b-antibody-oti3d1-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human endometrium tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-8.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human kidney tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-9.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human liver tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-10.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human lung carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-11.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human ovary adenocarcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-12.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human pancreas carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.com/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-13.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human breast thyroid carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.