Mouse Retinol binding protein 4 ELISA Kit (RBP4) (ab195459)
Key features and details
- Sensitivity: 6 ng/ml
- Range: 0.625 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Retinol binding protein 4 ELISA Kit (RBP4)
See all RBP4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5% Inter-assay Sample n Mean SD CV% Overall 7.2% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6 ng/ml -
Range
0.625 ng/ml - 20 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s Retinol binding protein 4 (RBP4) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP4 concentrations in mouse plasma, serum, urine and cell culture supernatants.
An RBP4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an RBP4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of RBP4 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse RBP4 Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Mouse RBP4 Microplate (12 x 8 well strips) 1 unit Mouse RBP4 Standard (Lyophilised) 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. -
Involvement in disease
Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- OTTHUMP00000020114
- OTTHUMP00000020115
- OTTHUMP00000020116
see all -
Database links
- Entrez Gene: 19662 Mouse
- SwissProt: Q00724 Mouse
- Unigene: 2605 Mouse
Images
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Typical Standard Curve
Representative standard curve using ab195459.
