Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.6 pg/ml
  • Range: 12.5 pg/ml - 800 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Retinol Binding Protein 4 ELISA Kit
    See all RBP4 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall 5 4.5%
    Inter-assay
    Sample n Mean SD CV%
    overall 3 3.8%

  • Sample type

    Cell culture supernatant, Urine, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.6 pg/ml

  • Range

    12.5 pg/ml - 800 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105 97% - 112%
    Urine 98 94% - 104%
    Serum 101 96% - 106%
    Hep Plasma 94 91% - 96%
    EDTA Plasma 99 94% - 103%
    Cit plasma 99 92% - 104%
    Tissue Homogenate 112 104% - 130%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Sheep, Goat, Cow, Pig

  • Product overview

    Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Retinol Binding Protein 4 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, urine, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Retinol Binding Protein 4 with 1.6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Retinol-Binding Protein 4 (RBP4) is a small, highly conserved member of the lipocalin superfamily involved in the transportation of retinol (Vitamin A) in blood. Synthesized in hepatocytes and adipocytes, RBP4 is also an adipokine involved in insulin resistance in the AG4KO mouse model. Elevated serum levels of RBP4 are seen in obesity and Type 2 diabetes.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)
    Other - Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Mouse RBP4 standard curve comparison data
    Mouse RBP4 standard curve comparison data

    Standard curve comparison between mouse RBP4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep kit shows a 25-fold increase in sensitivity.

  • Example of RBP4 standard curve prepared in Sample Diluent NS
    Example of RBP4 standard curve prepared in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of RBP4 standard curve prepared in 1X Cell Extraction Buffer PTR.
    Example of RBP4 standard curve prepared in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of mouse serum and plasma within the working range of the assay
    Titration of mouse serum and plasma within the working range of the assay

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Titration of mouse liver extract and mouse brain extract within the working range of the assay
    Titration of mouse liver extract and mouse brain extract within the working range of the assay

    Interpolated values of RBP4 are plotted for the indicated tissue extracts based on an extract load of 30 µg/mL. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Quantitation of RBP4 expression in different tissue culture supernatants
    Quantitation of RBP4 expression in different tissue culture supernatants

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)
    Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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