Mouse Retinol Binding Protein 4 Matched Antibody Pair Kit (ab214479)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 15.62 pg/ml - 1000 pg/ml
Overview
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Product name
Mouse Retinol Binding Protein 4 Matched Antibody Pair Kit
See all RBP4 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.94 pg/ml -
Range
15.62 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse RBP4 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse RBP4.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab202404. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Retinol Binding Protein 4 Capture Antibody 2 x 50µg 1 x 50µg Mouse Retinol Binding Protein 4 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Retinol Binding Protein 4 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. -
Involvement in disease
Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- OTTHUMP00000020114
- OTTHUMP00000020115
- OTTHUMP00000020116
see all -
Database links
- Entrez Gene: 19662 Mouse
- SwissProt: Q00724 Mouse
- Unigene: 2605 Mouse
Images
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Mouse Retinol Binding Protein 4 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Retinol Binding Protein 4 Matched Antibody Pair Kit (ab214479)To learn more about the advantages of recombinant antibodies see here.
