Mouse Retinol Binding Protein ELISA Kit (RBP) (ab190535)
Key features and details
- Sensitivity: 0.152 ng/ml
- Range: 0.625 ng/ml - 20 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Retinol Binding Protein ELISA Kit (RBP)
See all Retinol binding protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.152 ng/ml -
Range
0.625 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s Retinol Binding Protein (RBP) Mouse ELISA kit (ab190535) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Retinol Binding Protein (RBP) in mouse serum and plasma.
In this assay the Retinol Binding Protein (RBP) present in samples reacts with the anti-Retinol Binding Protein (RBP) antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-RBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound RBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of RBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of RBP in the test sample. The quantity of RBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml RBP Mouse Antibody Coated Microwells 1 unit RBP Mouse Calibrator (Lyophilised) 1 vial RBP Mouse HRP Conjugate 1 vial Stop Solution 1 x 12ml Chromogen Substrate Solution 1 x 12ml -
Research areas
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Database links
- SwissProt: P49194 Mouse
Images
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Standard Curve
Representative standard curve using ab190535 Retinol Binding Protein (RBP) Mouse ELISA Kit.
