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Anti-ASS1 antibody [2B10] (ab124465)

Key features and details

  • Mouse monoclonal [2B10] to ASS1
  • Suitable for: WB, ELISA, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-ASS1 antibody [2B10]
    See all ASS1 primary antibodies

  • Description

    Mouse monoclonal [2B10] to ASS1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Purified recombinant fragment of Human ASS1, expressed in E. Coli.

  • Positive control

    • ICC: HeLa cells. Flow Cyt: Jurkat cells. WB: Human ASS1 recombinant protein; HAP1, A431, Raji, L1210, MOLT4, Jurkat, A549, NIH 3T3, PC12 and COS7 cell lysates.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR119162-30 are from Tissue Culture Supernatant

Properties

Images

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: ASS1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124465 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab124465 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab124465 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-ASS1 antibody [2B10] (ab124465)
    Immunocytochemistry - Anti-ASS1 antibody [2B10] (ab124465)
    ab124465 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124465 at 1/1000 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
    ab124465, at 1/200 dilution, staining ASS1 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.
  • Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)
    Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)
    ab124465, at 1/200 dilution, staining ASS1 in Jurkat cells (green) by flow cytometry or negative control (red).
  • ELISA - Anti-ASS1 antibody [2B10] (ab124465)
    ELISA - Anti-ASS1 antibody [2B10] (ab124465)
    ELISA using ab124465.
  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    All lanes : Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : L1210 cell lysate
    Lane 4 : MOLT4 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : A549 cell lysate
    Lane 7 : NIH 3T3 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : COS7 cell lysate

    Predicted band size: 47 kDa

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution + Human ASS1 recombinant protein (aa: 40-236)

    Predicted band size: 47 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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