Anti-ASS1 antibody [2B10] (ab124465)
![Anti-ASS1 antibody [2B10] (ab124465)](https://www.abcam.com/ps/products/124/ab124465/Images/ab124465-282600-anti-ass1-antibody-2b10-western-blot.jpg "Anti-ASS1 antibody [2B10] (ab124465)")
Key features and details
- Mouse monoclonal [2B10] to ASS1
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-ASS1 antibody [2B10]
See all ASS1 primary antibodies -
Description
Mouse monoclonal [2B10] to ASS1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanICC/IF HumanWB Human
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Immunogen
Purified recombinant fragment of Human ASS1, expressed in E. Coli.
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Positive control
- ICC: HeLa cells. Flow Cyt: Jurkat cells. WB: Human ASS1 recombinant protein; HAP1, A431, Raji, L1210, MOLT4, Jurkat, A549, NIH 3T3, PC12 and COS7 cell lysates.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR119162-30 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2B10 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-ASS1 antibody [2B10] (ab124465)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: ASS1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab124465 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab124465 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab124465 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry - Anti-ASS1 antibody [2B10] (ab124465)](https://www.abcam.com/ps/products/124/ab124465/Images/ab124465-7-anti-ASS1-antibody-immunocytochemistry-immunofluorescence-HeLa-human-1.jpg)
Immunocytochemistry - Anti-ASS1 antibody [2B10] (ab124465)ab124465 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124465 at 1/1000 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)](https://www.abcam.com/ps/products/124/ab124465/Images/ab124465-138232-anti-ass1-antibody-2b10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)ab124465, at 1/200 dilution, staining ASS1 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. -
![Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)](https://www.abcam.com/ps/products/124/ab124465/Images/ab124465-138233-anti-ass1-antibody-2b10-flow-cytometry.jpg)
Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)ab124465, at 1/200 dilution, staining ASS1 in Jurkat cells (green) by flow cytometry or negative control (red). -
![ELISA - Anti-ASS1 antibody [2B10] (ab124465)](https://www.abcam.com/ps/products/124/ab124465/Images/ab124465-138234-anti-ass1-antibody-2b10-elisa.jpg)
ELISA - Anti-ASS1 antibody [2B10] (ab124465)ELISA using ab124465. -
![Western blot - Anti-ASS1 antibody [2B10] (ab124465)](https://www.abcam.com/ps/products/124/ab124465/Images/ab124465-138236-anti-ass1-antibody-2b10-western-blot.jpg)
Western blot - Anti-ASS1 antibody [2B10] (ab124465)All lanes : Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution
Lane 1 : A431 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : L1210 cell lysate
Lane 4 : MOLT4 cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : A549 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : COS7 cell lysate
Predicted band size: 47 kDa
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![Western blot - Anti-ASS1 antibody [2B10] (ab124465)](https://www.abcam.com/ps/products/124/ab124465/Images/ab124465-138235-anti-ass1-antibody-2b10-western-blot.jpg)
Western blot - Anti-ASS1 antibody [2B10] (ab124465)Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution + Human ASS1 recombinant protein (aa: 40-236)
Predicted band size: 47 kDa
