Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
![Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)](https://www.abcam.com/ps/products/170/ab170900/Images/ab170900-448861-anti-ass1-antibody-epr12399b-c-terminal-western-blot-hela-lysates.jpg "Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12399(B)] to ASS1 - C-terminal
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ASS1 antibody [EPR12399(B)] - C-terminal
See all ASS1 primary antibodies -
Description
Rabbit monoclonal [EPR12399(B)] to ASS1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human ASS1 aa 350 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P00966 -
Positive control
- WB: HAP1, HeLa, Jurkat and fetal liver cell lysates. IP: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12399(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)All lanes : Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ASS1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDaLanes 1- 2: Merged signal (red and green). Green - ab170900 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab170900 was shown to react with ASS1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264989 (knockout cell lysate ab257143) was used. Wild-type HeLa and ASS1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab170900 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)](https://www.abcam.com/ps/products/170/ab170900/Images/ab170900-282599-anti-ass1-antibody-epr12399b-c-terminal-western-blot.jpg)
Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)All lanes : Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ASS1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDaLanes 1 - 4: Merged signal (red and green). Green - ab170900 observed at 47 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab170900 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab170900 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)](https://www.abcam.com/ps/products/170/ab170900/Images/ab170900-172101-anti-ass1-antibody-epr12399b-c-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1X PBS (negative control) using ab170900 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)](https://www.abcam.com/ps/products/170/ab170900/Images/ab170900-172102-anti-ass1-antibody-epr12399b-c-terminal-western-blot.jpg)
Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)All lanes : Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
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![Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)](https://www.abcam.com/ps/products/170/ab170900/Images/ab170900-5-benefits-of-recombinant-antibodies.png)
Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
