Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12399(B)] to ASS1 - C-terminal
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ASS1 antibody [EPR12399(B)] - C-terminal
    See all ASS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12399(B)] to ASS1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ASS1 aa 350 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P00966

  • Positive control

    • WB: HAP1, HeLa, Jurkat and fetal liver cell lysates. IP: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
    Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
    All lanes : Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ASS1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab170900 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab170900 was shown to react with ASS1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264989 (knockout cell lysate ab257143) was used. Wild-type HeLa and ASS1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab170900 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
    Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
    All lanes : Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ASS1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : Jurkat whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 47 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab170900 observed at 47 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab170900 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab170900 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
    Immunoprecipitation - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)

    Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1X PBS (negative control) using ab170900 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
    Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
    All lanes : Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa

  • Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)
    Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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