Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2987(2)(ABC)(B)] to C3 / C3a
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)]
    See all C3 / C3a primary antibodies

  • Description

    Rabbit monoclonal [EPR2987(2)(ABC)(B)] to C3 / C3a

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human C3/ C3a aa 850-950. The exact sequence is proprietary.
    Database link: P01024

  • Positive control

    • Human plasma, fetal liver, fetal kidney, HepG2 and Human milk lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR2987(2)(ABC)(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080)
    Western blot - Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080)
    All lanes : Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080) at 1/1000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human fetal kidney tissue lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : Human milk lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 187 kDa

  • Immunoprecipitation - Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080)
    Immunoprecipitation - Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080)

    Western blot analysis on immunoprecipitation pellet from fetal kidney lysate (Lane 1) or 1X PBS (negative control) (Lane 2) labeling C3 / C3a using ab171080 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    Lane 1: Primary: ab171080, 1/1000 dilution
    Sample: Fetal kidney tissue lysate
    Secondary: HRP-conjugated anti-rabbit IgG

    Lane 2: Primary: ab171080, 1/1000 dilution
    Sample: 1 X PBS
    Secondary: HRP-conjugated anti-rabbit IgG

  • Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080)
    Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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