Recombinant mouse AMPK alpha 1 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) (ab268344)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
-
Product name
Recombinant mouse AMPK alpha 1 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) -
Biological activity
The specific activity of ab268344 was 340 nmol/min/min in a peptide kinase assay using SAMStide (HMRSAMSGLHLVKRR) as substrate.
-
Purity
> 75 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Amino Acid Sequence 1
-
Species
Mouse -
Sequence
MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTG HKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDI FMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRD LKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRL YAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNP SVISLLKHMLQVDPMKRAAIKDIREHEWFKQDLPKYLFPEDPSYSSTMID DEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKD FYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQ GVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNP VTSTFSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSISNY RSCQRSDSDAEAQGKPSDVSLTSSVTSLDSSPVDVAPRPGSHTIEFFEMC ANLIKILAQ -
Molecular weight information
SDS-PAGE molecular weight: ~68-72 kDa -
Amino acids
1 to 559 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_001013367
-
Amino Acid Sequence 2
-
Species
Mouse -
Sequence
MGNTTSERVSGERHGAKAARAEGGGHGPGKEHKIMVGSTDDPSVFSLPDS KLPGDKEFVPWQQDLDDSVKPAQQARPTVIRWSEGGKEVFISGSFNNWST KIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINN LIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEER FKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVM VLSATHRYKKKYVTTLLYKPI -
Molecular weight information
SDS-PAGE molecular weight: ~35 kDa -
Amino acids
1 to 271 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_182997
-
Amino Acid Sequence 3
-
Species
Mouse -
Sequence
MESVAAESSPALENEHFQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVF DTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKS ALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIH RLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLQELQIGTY ANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAA EKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLV VVDEHDVVKGIVSLSDILQALVLTGGEKKP -
Molecular weight information
SDS-PAGE molecular weight: ~40 kDa -
Amino acids
1 to 330 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_016781
-
Preparation and Storage
Images
-
Functional Studies - Recombinant mouse AMPK alpha 1 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) (ab268344)
The specific activity of ab268344 was 340 nmol/min/min in a peptide kinase assay using SAMStide (HMRSAMSGLHLVKRR) as substrate.
-
SDS-PAGE - Recombinant mouse AMPK alpha 1 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) (ab268344)SDS-PAGE analysis of ab268344.