Anti-fast skeletal Myosin antibody (ab228727)
Key features and details
- Rabbit polyclonal to fast skeletal Myosin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-fast skeletal Myosin antibody
See all fast skeletal Myosin primary antibodies -
Description
Rabbit polyclonal to fast skeletal Myosin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Pig
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Immunogen
Recombinant fragment within Human fast skeletal Myosin (internal sequence). The exact sequence is proprietary.
Database link: P05976 -
Positive control
- WB: HeLa whole cell lysate; Mouse heart lysate; Rat muscle lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-fast skeletal Myosin antibody (ab228727)
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for fast skeletal Myosin (green) using ab228727 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-fast skeletal Myosin antibody (ab228727)Anti-fast skeletal Myosin antibody (ab228727) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa15% SDS-PAGE gel.
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Western blot - Anti-fast skeletal Myosin antibody (ab228727)All lanes : Anti-fast skeletal Myosin antibody (ab228727) at 1/10000 dilution
Lane 1 : Mouse heart lysate at 1 µg
Lane 2 : Mouse heart lysate at 5 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa15% SDS-PAGE gel.
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Western blot - Anti-fast skeletal Myosin antibody (ab228727)Anti-fast skeletal Myosin antibody (ab228727) at 1/10000 dilution + Rat muscle lysate at 1 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa15% SDS-PAGE gel.
