Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)
![Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)](https://www.abcam.com/ps/products/89/ab89594/Images/ab89594-4-ab89594FC.jpg "Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)")
Key features and details
- Mouse monoclonal [AT3B2] to Myosin Light Chain 2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Myosin Light Chain 2 antibody [AT3B2]
See all Myosin Light Chain 2 primary antibodies -
Description
Mouse monoclonal [AT3B2] to Myosin Light Chain 2 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Myosin Light Chain 2 aa 1-166.
Database link: P10916 -
Positive control
- Rat heart extracts. Flow Cyt (Intra).:A431 cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 11th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.2% Sodium azide
Constituents: PBS, 10% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT3B2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)
Overlay histogram showing A431 cells stained with ab89594 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89594, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)](https://www.abcam.com/ps/products/89/ab89594/Images/Myosin-Light-Chain-2-Primary-antibodies-ab89594-1.jpg)
Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/1000 dilution + rat heart extracts at 60 µg
Secondary
goat anti-mouse HRP
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 19 kDaThis image was generated using the ascites version of the product.
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![Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)](https://www.abcam.com/ps/products/89/ab89594/Images/Myosin-Light-Chain-2-Primary-antibodies-ab89594-4.jpg)
Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) This image is courtesy of an anonymous AbreviewAnti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/2000 dilution + Rabbit heart tissue lysate at 10 µg
Secondary
HRP conjugated goat anti-mouse at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 1 minuteThis image was generated using the ascites version of the product.
