Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C3 / C3a antibody [2898] (ab11874)

Key features and details

  • Mouse monoclonal [2898] to C3 / C3a
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-C3 / C3a antibody [2898]
    See all C3 / C3a primary antibodies

  • Description

    Mouse monoclonal [2898] to C3 / C3a

  • Host species

    Mouse

  • Specificity

    This antibody reacts with the C-terminus of C3a. It reacts with intact C3 as with C3a.

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide, corresponding to amino acids

    CARASHLGLA

    of C3/ C3a (last nine amino acids of C3a-desArg).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human plasma

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    0.2 µm filtered

  • Clonality

    Monoclonal

  • Clone number

    2898

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-C3 / C3a antibody [2898] (ab11874)
    Western blot - Anti-C3 / C3a antibody [2898] (ab11874)
    All lanes : Anti-C3 / C3a antibody [2898] (ab11874) at 1/200 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 187.2 kDa
    Observed band size: 187 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 115 kDa, 71 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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