Anti-C3 / C3a antibody [2898] (ab11874)
![Anti-C3 / C3a antibody [2898] (ab11874)](https://www.abcam.com/ps/products/11/ab11874/Images/ab11874-19125-anti-c3-c3a-antibody-2898-western-blot.jpg "Anti-C3 / C3a antibody [2898] (ab11874)")
Key features and details
- Mouse monoclonal [2898] to C3 / C3a
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-C3 / C3a antibody [2898]
See all C3 / C3a primary antibodies -
Description
Mouse monoclonal [2898] to C3 / C3a -
Host species
Mouse -
Specificity
This antibody reacts with the C-terminus of C3a. It reacts with intact C3 as with C3a. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to amino acids
CARASHLGLA
of C3/ C3a (last nine amino acids of C3a-desArg). -
Positive control
- Human plasma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Clonality
Monoclonal -
Clone number
2898 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-C3 / C3a antibody [2898] (ab11874)All lanes : Anti-C3 / C3a antibody [2898] (ab11874) at 1/200 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 187.2 kDa
Observed band size: 187 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 71 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
