Anti-PWP1 antibody [EPR16130] (ab190794)
![Anti-PWP1 antibody [EPR16130] (ab190794)](https://www.abcam.com/ps/products/190/ab190794/Images/ab190794-226979-anti-pwp1-antibody-epr16130-western-blot.jpg "Anti-PWP1 antibody [EPR16130] (ab190794)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16130] to PWP1
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-PWP1 antibody [EPR16130]
See all PWP1 primary antibodies -
Description
Rabbit monoclonal [EPR16130] to PWP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HeLa, 293, HepG2 cell lysates, Human pancreas tissue, Jurkat cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16130 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PWP1 antibody [EPR16130] (ab190794)Anti-PWP1 antibody [EPR16130] (ab190794) at 1/10000 dilution + Jurkat cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Additional bands at: 79 kDa. We are unsure as to the identity of these extra bands.
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![Western blot - Anti-PWP1 antibody [EPR16130] (ab190794)](https://www.abcam.com/ps/products/190/ab190794/Images/ab190794-226978-anti-pwp1-antibody-epr16130-western-blot.jpg)
Western blot - Anti-PWP1 antibody [EPR16130] (ab190794)All lanes : Anti-PWP1 antibody [EPR16130] (ab190794) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PWP1 antibody [EPR16130] (ab190794)](https://www.abcam.com/ps/products/190/ab190794/Images/ab190794-226977-anti-pwp1-antibody-epr16130-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PWP1 antibody [EPR16130] (ab190794)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling PWP1 with ab190794 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-PWP1 antibody [EPR16130] (ab190794)](https://www.abcam.com/ps/products/190/ab190794/Images/ab190794-226976-anti-pwp1-antibody-epr16130-flow-cytometry.jpg)
Flow Cytometry - Anti-PWP1 antibody [EPR16130] (ab190794)Flow cytometry analysis of PWP1 expression in Jurkat cells using ab190794 at 1/30 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
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![Anti-PWP1 antibody [EPR16130] (ab190794)](https://www.abcam.com/ps/products/190/ab190794/Images/ab190794-5-benefits-of-recombinant-antibodies.png)
Anti-PWP1 antibody [EPR16130] (ab190794)
