Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-PWP1 antibody [EPR16130] (ab190794)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16130] to PWP1
Suitable for: IHC-P, Flow Cyt, WB
Reacts with: Human

Product name

Anti-PWP1 antibody [EPR16130]
See all PWP1 primary antibodies
Description

Rabbit monoclonal [EPR16130] to PWP1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat, HeLa, 293, HepG2 cell lysates, Human pancreas tissue, Jurkat cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16130
Isotype

IgG
Research areas

Western blot - Anti-PWP1 antibody [EPR16130] (ab190794)

Anti-PWP1 antibody [EPR16130] (ab190794) at 1/10000 dilution + Jurkat cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Additional bands at: 79 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-PWP1 antibody [EPR16130] (ab190794)

All lanes : Anti-PWP1 antibody [EPR16130] (ab190794) at 1/1000 dilution

Lane 1 : Hela cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PWP1 antibody [EPR16130] (ab190794)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling PWP1 with ab190794 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-PWP1 antibody [EPR16130] (ab190794)

Flow cytometry analysis of PWP1 expression in Jurkat cells using ab190794 at 1/30 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
Anti-PWP1 antibody [EPR16130] (ab190794)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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