Anti-HSPC111 antibody (ab241590)
Key features and details
- Rabbit polyclonal to HSPC111
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSPC111 antibody
See all HSPC111 primary antibodies -
Description
Rabbit polyclonal to HSPC111 -
Host species
Rabbit -
Specificity
Antibody was affinity purified using an epitope specific to HSPC111 immobilized on solid support. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide within Human HSPC111 aa 75-125. The exact sequence is proprietary.
Database link: Q9Y3C1 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to HSPC111 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HSPC111 antibody (ab241590)All lanes : Anti-HSPC111 antibody (ab241590) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 21 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-HSPC111 antibody (ab241590)HSPC111 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241590 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241590 at 0.4 µg/mL.
Lane 1: ab241590 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
Lysates prepared in NETN buffer.
