Anti-JunD antibody (ab74428)
Key features and details
- Rabbit polyclonal to JunD
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-JunD antibody
See all JunD primary antibodies -
Description
Rabbit polyclonal to JunD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human JunD aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab74427) -
Positive control
- This antibody gave a positive signal in the following Human Nuclear Lysates: HeLa, Jurkat
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications CowDogChimpanzeeApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).Target
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Function
Transcription factor binding AP-1 sites. -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3727 Human
- Omim: 165162 Human
- SwissProt: P17535 Human
- Unigene: 2780 Human
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Images
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Western blot - Anti-JunD antibody (ab74428)All lanes : Anti-JunD antibody (ab74428) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The 43 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to JunD.
Datasheets and documents
References (0)
ab74428 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-JunD antibody (ab74428)All lanes : Anti-JunD antibody (ab74428) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The 43 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to JunD.
