Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JunD antibody [EPR6520] (ab134067)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6520] to JunD
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-JunD antibody [EPR6520]
    See all JunD primary antibodies

  • Description

    Rabbit monoclonal [EPR6520] to JunD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human JunD aa 100-200. The exact sequence is proprietary.

  • Positive control

    • 293T, Jurkat, HepG2 and HeLa whole cell lysate (ab150035); Human skin and kidney tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-JunD antibody [EPR6520] (ab134067)
    Western blot - Anti-JunD antibody [EPR6520] (ab134067)
    Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,43 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (ab134067)
    Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (ab134067)

    Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

  • Immunoprecipitation - Anti-JunD antibody [EPR6520] (ab134067)
    Immunoprecipitation - Anti-JunD antibody [EPR6520] (ab134067)

    ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-JunD antibody [EPR6520] (ab134067)
    Western blot - Anti-JunD antibody [EPR6520] (ab134067)
    All lanes : Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,43 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (ab134067)
    Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (ab134067)

    Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, unpurified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

  • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (ab134067)
    Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (ab134067)

    Immunofluorescent analysis of HeLa cells labelling JunD with unpurified ab134067 at 1/100 dilution.

  • Immunoprecipitation - Anti-JunD antibody [EPR6520] (ab134067)
    Immunoprecipitation - Anti-JunD antibody [EPR6520] (ab134067)

    ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • OI-RD Scanning - Anti-JunD antibody [EPR6520] (ab134067)
    OI-RD Scanning - Anti-JunD antibody [EPR6520] (ab134067)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-JunD antibody [EPR6520] (ab134067)
    Anti-JunD antibody [EPR6520] (ab134067)

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