Anti-STAT1 alpha antibody (ab2071)
Key features and details
- Rabbit polyclonal to STAT1 alpha
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-STAT1 alpha antibody
See all STAT1 alpha primary antibodies -
Description
Rabbit polyclonal to STAT1 alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human STAT1 alpha aa 712-750. The sequences differ from the murine corresponding sequences by four amino acids.
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General notes
JAK activated transcription.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
DEAE-Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 alpha antibody (ab2071)
Immunofluorescence of STAT1 in human colon tissue with ab2071 at 20 μg/mL.
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Western blot - Anti-STAT1 alpha antibody (ab2071)All lanes : Anti-STAT1 alpha antibody (ab2071) at 0.5 µg/ml
Lane 1 : Mouse skin
Lane 2 : Mouse skeletal muscle
Lane 3 : Mouse bladder
Lane 4 : Mouse lung
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 100 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 alpha antibody (ab2071)Immunohistochemistry of STAT1 in human colon tissue with ab2071 at 2.5 μg/ml.
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Western blot - Anti-STAT1 alpha antibody (ab2071)Anti-STAT1 alpha antibody (ab2071) at 1 µg/ml + YB2/0 at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 100 kDa
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Western blot - Anti-STAT1 alpha antibody (ab2071)All lanes : Anti-STAT1 alpha antibody (ab2071) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : K562 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Predicted band size: 100 kDa
Observed band size: 91 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-STAT1 alpha antibody (ab2071)Immunocytochemistry of STAT1a in HeLa cells with STAT1a antibody at 10 µg/ml.
