Anti-ATF-4 antibody [N360A/24] (ab186284)
![Anti-ATF-4 antibody [N360A/24] (ab186284)](https://www.abcam.com/ps/products/186/ab186284/Images/ab186284-312724-anti-atf-4-antibody-n360a-24-immunofluorescence.jpg "Anti-ATF-4 antibody [N360A/24] (ab186284)")
Key features and details
- Mouse monoclonal [N360A/24] to ATF-4
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2a
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N360A/24 -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ATF-4 antibody [N360A/24] (ab186284)
SK-N-BE (Human neuroblastoma cell line) cells stained for ATF-4 (Green) using ab186284 at a 1/100 dilution (1 hour at RT) in ICC/IF.
Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.
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![Western blot - Anti-ATF-4 antibody [N360A/24] (ab186284)](https://www.abcam.com/ps/products/186/ab186284/Images/ab186284-312723-anti-atf-4-antibody-n360a-24-western-blot.jpg)
Western blot - Anti-ATF-4 antibody [N360A/24] (ab186284)Anti-ATF-4 antibody [N360A/24] (ab186284) at 1/2000 dilution + Rat brain lysate at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 5 minutesBlock: 5% Skimmed milk in 1X TBST.
Primary antibody incubated for 2 hours at RT.
Secondary antibody incubated for 1 hour at RT.
