Anti-ATF-4 antibody (ab23760)
Key features and details
- Rabbit polyclonal to ATF-4
- Suitable for: ICC/IF, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-ATF-4 antibody
See all ATF-4 primary antibodies -
Description
Rabbit polyclonal to ATF-4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Recombinant fragment
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Immunogen
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Positive control
- ATF-4 tagged recombinant protein This antibody gave a positive signal in the following Methanol fixed cell lines: MCF-7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB Recombinant fragmentAll applications MouseRatApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).Target
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Function
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localization
Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes. - Information by UniProt
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Database links
- Entrez Gene: 468 Human
- Entrez Gene: 11911 Mouse
- Entrez Gene: 79255 Rat
- Omim: 604064 Human
- SwissProt: P18848 Human
- SwissProt: Q06507 Mouse
- SwissProt: Q9ES19 Rat
- Unigene: 496487 Human
see all -
Alternative names
- Activating transcription factor 4 antibody
- ATF 4 antibody
- ATF4 antibody
see all
Images
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Western blot - Anti-ATF-4 antibody (ab23760)Anti-ATF-4 antibody (ab23760) at 1 µg/ml + ATF4 tagged recombinant protein at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Ab23760 gave a positive signal against the tagged recombinant ATF4 protein which is known to run at 76 kDa. -
Immunocytochemistry/ Immunofluorescence - Anti-ATF-4 antibody (ab23760)ICC/IF image of ab23760 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23760 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References (26)
ab23760 has been referenced in 26 publications.
- Zhu X et al. lncRNA MEG3 promotes hepatic insulin resistance by serving as a competing endogenous RNA of miR-214 to regulate ATF4 expression. Int J Mol Med 43:345-357 (2019). PubMed: 30431065
- Lin J et al. Berberine, a Traditional Chinese Medicine, Reduces Inflammation in Adipose Tissue, Polarizes M2 Macrophages, and Increases Energy Expenditure in Mice Fed a High-Fat Diet. Med Sci Monit 25:87-97 (2019). PubMed: 30606998
- Huo JF & Chen XB P2X4R silence suppresses glioma cell growth through BDNF/TrkB/ATF4 signaling pathway. J Cell Biochem 120:6322-6329 (2019). PubMed: 30362154
- Cui Y et al. Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2-PERK pathway. Int J Mol Med 43:2015-2023 (2019). PubMed: 30864682
- Zhou XT et al. Inhibition of autophagy enhances adenosine-induced apoptosis in human hepatoblastoma HepG2 cells. Oncol Rep 41:829-838 (2019). PubMed: 30535464
Images
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Western blot - Anti-ATF-4 antibody (ab23760)Anti-ATF-4 antibody (ab23760) at 1 µg/ml + ATF4 tagged recombinant protein at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Ab23760 gave a positive signal against the tagged recombinant ATF4 protein which is known to run at 76 kDa. -
Immunocytochemistry/ Immunofluorescence - Anti-ATF-4 antibody (ab23760)
ICC/IF image of ab23760 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23760 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
