PE Anti-ATF-4 antibody [EPR18111] (ab225333)
![PE Anti-ATF-4 antibody [EPR18111] (ab225333)](https://www.abcam.com/ps/products/225/ab225333/Images/ab225333-310815-anti-atf-4-antibody-epr18111-phycoerythrin-flow-cytometry.jpg "PE Anti-ATF-4 antibody [EPR18111] (ab225333)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR18111] to ATF-4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-ATF-4 antibody [EPR18111]
See all ATF-4 primary antibodies -
Description
PE Rabbit monoclonal [EPR18111] to ATF-4 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment within Human ATF-4 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P18848 -
Positive control
- Flow Cyt: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18111 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - PE Anti-ATF-4 antibody [EPR18111] (ab225333)
Overlay histogram showing HeLa cells stained with ab225333 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225333, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-ATF-4 antibody [EPR18111] (ab225333)](https://www.abcam.com/ps/products/225/ab225333/Images/ab225333-3-benefits-of-recombinant-antibodies.png)
PE Anti-ATF-4 antibody [EPR18111] (ab225333)
