Anti-ESE1 antibody (ab275592)
Key features and details
- Rabbit polyclonal to ESE1
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ESE1 antibody
See all ESE1 primary antibodies -
Description
Rabbit polyclonal to ESE1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ESE1 (C terminal).
Database link: P78545 -
Positive control
- WB: A431 whole cell lysate. ICC: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS
0.2 µm filtered solution -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A & Antigen Affinity -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ESE1 antibody (ab275592)Anti-ESE1 antibody (ab275592) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Observed band size: 41 kDa why is the actual band size different from the predicted?Reducing conditions.
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Immunocytochemistry - Anti-ESE1 antibody (ab275592)Immunofluorescence analysis of A431 (human epidermoid carcinoma cell line) cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab275592 at 1/5000 dilution at 4° overnight. Then cells were stained with an Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.