Anti-POMC antibody (ab32893)
Key features and details
- Goat polyclonal to POMC
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-POMC antibody
See all POMC primary antibodies -
Description
Goat polyclonal to POMC -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human POMC aa 256-267 (C terminal) (Cysteine residue).
Sequence:C-NAIIKNAYKKGE
Database link: P01189 -
Positive control
- WB: NIH/3T3 cell lysate and rat brain tissue lysate; IHC-P: Human pituitary gland; Flow Cyt (intra): A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POMC antibody (ab32893)Lane 1 : Anti-POMC antibody (ab32893) at 0.3 µg/ml
Lane 2 : Anti-POMC antibody (ab32893) at 2 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 29 kDaPrimary incubation 1 hour at room temperature.
Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)Immunohistochemical analysis of Human pituitary gland labeling POMC with ab32893. Antigen retrieval by steaming with Tris/EDTA buffer pH 9. HRP-staining. -
Flow Cytometry - Anti-POMC antibody (ab32893)Flow cytometry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells (blue line) labeling POMC with ab32893 at 10 µg/mL. Cells permeabilized with 0.5% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 1 µg/mL.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
