Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17571] to POMC - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-POMC antibody [EPR17571] - BSA and Azide free
    See all POMC primary antibodies

  • Description

    Rabbit monoclonal [EPR17571] to POMC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with POMC whole cell lysate; rat and mouse pituitary lysates; human ACTH recombinant protein. IHC-P: Human, mouse and rat pituitary tissues. IP: Rat pituitary whole cell lysate.

  • General notes

    Ab222486 is the carrier-free version of ab210605. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab222486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Immunoprecipitation - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

    ab210605 at 1/80 immunoprecipitating POMC in Rat pituitary whole cell lysate observed at 29 KDa (lanes 1 and 2).

    Lane 1 (input): Rat pituitary whole cell lysate 10μg

    Lane 2 (+): ab210605 + Rat pituitary whole cell lysate 

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab210605 in Rat pituitary whole cell lysate

    For western blotting, ab210605 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used for detection at 1/10000 dilution.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210605).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on human pituitary is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210605).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative staining on human kidney.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210605).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative staining on human colon cancer.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210605).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on mouse pituitary is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210605).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative staining on mouse kidney.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210605).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

    Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on rat pituitary is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210605).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative staining on rat liver.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210605).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)
    Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-POMC antibody [EPR17571] - BSA and Azide free (ab222486)

© 2021 Astana Biomed Group LLP. All rights reserved.