Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22534-165] to POMC - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-POMC antibody [EPR22534-165] - BSA and Azide free
    See all POMC primary antibodies

  • Description

    Rabbit monoclonal [EPR22534-165] to POMC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse, human and rat pituitary tissue lysate. IHC-P: Mouse pituitary and spleen; human and rat pituitary tissue. IHC-Fr: Mouse and rat pituitary and liver tissue.

  • General notes

    ab255334 is the carrier-free version of ab254257.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labelling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pituitary is observed (PMID: 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

    Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pituitary is observed (PMID: 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pituitary is observed (PMID: 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

    Negative control: No staining on mouse spleen (PMID: 10395956)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

  • Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

    Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed mouse pituitary tissue labeling POMC with ab254257 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Positive staining on mouse pituitary (PMID: 18601691).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

  • Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

    Negative control: No stain on liver (PMID: 14578285)

    Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed rat liver tissue labeling POMC with ab254257 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. No staining on rat liver.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

  • Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

    Negative control: No stain on liver (PMID: 14578285)

    Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed mouse liver tissue labeling POMC with ab254257 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. No staining on mouse liver.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

  • Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

    Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed rat pituitary tissue labeling POMC with ab254257 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Positive staining on rat pituitary (PMID: 18601691).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

  • Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)
    Anti-POMC antibody [EPR22534-165] - BSA and Azide free (ab255334)

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