Human TIMP4 ELISA Kit (ab113328)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 28.67 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TIMP4 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
28.67 pg/ml - 7000 pg/mlRecovery
80 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.11 89% - 104% Serum 73.49 67% - 82% Plasma 72.83 67% - 81% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s TIMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP4 in serum, plasma (collect plasma using EDTA and citrate as an anticoagulant. Heparin is not recommended), cell culture supernatants.
This assay employs an antibody specific for Human TIMP4 coated on a 96- well plate. Standards and samples are pipetted into the wells and TIMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Notes
Optimization may be required with urine samples.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human TIMP4 2 vials Recombinant Human TIMP4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TIMP4 Microplate (12 strips x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Alternative names
- Metalloproteinase inhibitor 4
- TIMP 4
- TIMP metallopeptidase inhibitor 4
see all -
Database links
- Entrez Gene: 7079 Human
- Omim: 601915 Human
- SwissProt: Q99727 Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human TIMP4 2 vials Recombinant Human TIMP4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TIMP4 Microplate (12 strips x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Alternative names
- Metalloproteinase inhibitor 4
- TIMP 4
- TIMP metallopeptidase inhibitor 4
see all -
Database links
- Entrez Gene: 7079 Human
- Omim: 601915 Human
- SwissProt: Q99727 Human
Images
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Representative Standard Curve
Representative Standard Curve using ab113328
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Representative Standard CurveRepresentative Standard Curve using ab113328