Anti-Interferon gamma antibody (ab9657)
Key features and details
- Rabbit polyclonal to Interferon gamma
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Interferon gamma antibody
See all Interferon gamma primary antibodies -
Description
Rabbit polyclonal to Interferon gamma -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human Interferon gamma. Mature Interferon gamma is from aa 24-161 of SwissProt ID Q14609. aa 1-23 represents the signal peptide.
Database link: Q14609 -
Positive control
- Purchase matching WB positive control:Recombinant human Interferon gamma protein
- WB: Recombinant human Interferon gamma protein (ab9659). Sandwich ELISA: Recombinant human Interferon gamma protein (ab9659).
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab231036.
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
affinity chromatography employing immobilized Human IFN-gamma matrix. -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-Interferon gamma antibody (ab9657)Lanes 2-12 : Anti-Interferon gamma antibody (ab9657) at 0.1 µg/ml
Lane 1 : Marker
Lanes 2-12 :Recombinant human Interferon gamma protein (Active) (ab9659)
Performed under non-reducing conditions.
Predicted band size: 17 kDa -
Sandwich ELISA - Anti-Interferon gamma antibody (ab9657)To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with a compatible secondary detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ.
