Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Interferon gamma antibody [EPR21704] (ab231036)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21704] to Interferon gamma
Suitable for: WB, IHC-P, Flow Cyt, ELISA
Reacts with: Human, Recombinant fragment

Product name

Anti-Interferon gamma antibody [EPR21704]
See all Interferon gamma primary antibodies
Description

Rabbit monoclonal [EPR21704] to Interferon gamma
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NK-92 treated with PMA (ab120297), Ionomycin and BFA, cell lysate. IHC-P: Human tonsil and NK-92 cells treated with PMA (ab120297), Ionomycin and BFA, cell lysate. Flow: NK-92 treated treated with PMA (ab120297), Ionomycin and BFA, cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21704
Isotype

IgG
Research areas

Flow Cytometry - Anti-Interferon gamma antibody [EPR21704] (ab231036)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cell line treated with 80 μM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 μM Ionomycin for 5 hours, then 300 ng/ml BFA was added for 4 hours labeling Interferon gamma with ab231036 at 1/60 (red) and untreated control (green). Compared with a Rabbit monoclonal IgG-Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary anibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Interferon gamma with ab231036 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Sporadic cytoplasmic staining in limphocytes of human tonsil is observed (PMID: 21838712). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Interferon gamma antibody [EPR21704] (ab231036)

All lanes : Anti-Interferon gamma antibody [EPR21704] (ab231036) at 1/1000 dilution

Lane 1 : Untreated NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell), whole cell lysate.
Lane 2 : NK-92 treated with 80µM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 µM Ionomycin for 5 hours, then added 300 ng/ml BFA for 4 hours.

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 19 kDa
Observed band size: 20 kDa
why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking/dilution buffer and concentration: 5% NFDM/TBST

Expression of IFN Gamma in NK-92 could be induced by PMA and Ionomycin treatment according to the literature (PMID: 23129404).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)

Immunohistochemical analysis of paraffin-embedded NK-92 cells labeling Interferon gamma with ab231036 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Nearly no staining on untreated NK92 cells (A) and positive staining on treated NK92 cells (B) (treated with 80 μM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 μM Ionomycin for 5 hours, then with 300 ng/ml BFA for 4 hours) is observed (PMID:23129404). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
ELISA - Anti-Interferon gamma antibody [EPR21704] (ab231036)

ELISA analysis of Human IFN Gamma recombinant protein at 1000 ng/mL with ab231036. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Anti-Interferon gamma antibody [EPR21704] (ab231036)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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