Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon gamma antibody [EPR21704] (ab231036)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21704] to Interferon gamma
  • Suitable for: WB, IHC-P, Flow Cyt, ELISA
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Interferon gamma antibody [EPR21704]
    See all Interferon gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR21704] to Interferon gamma

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92 treated with PMA (ab120297), Ionomycin and BFA, cell lysate. IHC-P: Human tonsil and NK-92 cells treated with PMA (ab120297), Ionomycin and BFA, cell lysate. Flow: NK-92 treated treated with PMA (ab120297), Ionomycin and BFA, cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Interferon gamma antibody [EPR21704] (ab231036)
    Flow Cytometry - Anti-Interferon gamma antibody [EPR21704] (ab231036)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cell line treated with 80 μM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 μM Ionomycin for 5 hours, then 300 ng/ml BFA was added for 4 hours labeling Interferon gamma with ab231036 at 1/60 (red) and untreated control (green). Compared with a Rabbit monoclonal IgG-Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary anibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Interferon gamma with ab231036 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Sporadic cytoplasmic staining in limphocytes of human tonsil is observed (PMID: 21838712). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Interferon gamma antibody [EPR21704] (ab231036)
    Western blot - Anti-Interferon gamma antibody [EPR21704] (ab231036)
    All lanes : Anti-Interferon gamma antibody [EPR21704] (ab231036) at 1/1000 dilution

    Lane 1 : Untreated NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell), whole cell lysate.
    Lane 2 : NK-92 treated with 80µM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 µM Ionomycin for 5 hours, then added 300 ng/ml BFA for 4 hours.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 19 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    Expression of IFN Gamma in NK-92 could be induced by PMA and Ionomycin treatment according to the literature (PMID: 23129404).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)

    Immunohistochemical analysis of paraffin-embedded NK-92 cells labeling Interferon gamma with ab231036 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Nearly no staining on untreated NK92 cells (A) and positive staining on treated NK92 cells (B) (treated with 80 μM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 μM Ionomycin for 5 hours, then with 300 ng/ml BFA for 4 hours) is observed (PMID:23129404). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • ELISA - Anti-Interferon gamma antibody [EPR21704] (ab231036)
    ELISA - Anti-Interferon gamma antibody [EPR21704] (ab231036)
    ELISA analysis of Human IFN Gamma recombinant protein at 1000 ng/mL with ab231036. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-Interferon gamma antibody [EPR21704] (ab231036)
    Anti-Interferon gamma antibody [EPR21704] (ab231036)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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