Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free
    See all Interferon alpha/beta receptor 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247992 is the carrier-free version of ab124764 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247992 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : Interferon alpha/beta receptor 1 knockout HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 64 kDa



    This data was developed using ab124764, the same antibody clone in a different buffer formulation.

    Lanes 1 - 3: Merged signal (red and green). Green - ab124764 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab124764 was shown to specifically react with Interferon Receptor alpha in wild-type HeLa cells as signal was lost in Interferon alpha/beta receptor 1 knockout cells. Wild-type and Interferon alpha/beta receptor 1 knockout samples were subjected to SDS-PAGE. ab124764 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 (ab124764) at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab124764, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

    Lane 1 : HEK-293 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : LNCaP whole cell lysate
    Lane 4 : SH-SY5Y whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 64 kDa
    Observed band size: 90-130 kDa why is the actual band size different from the predicted?



    This data was developed using ab124764, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse brain whole tissue lysate
    Lane 2 : Mouse heart whole tissue lysate
    Lane 3 : Mouse kidney whole tissue lysate
    Lane 4 : Mouse spleen whole tissue lysate
    Lane 5 : Rat brain whole tissue lysate
    Lane 6 : Rat heart whole cell lysate
    Lane 7 : Rat kidney whole cell lysate
    Lane 8 : Rat spleen whole tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated at 1/2000 dilution

    Predicted band size: 64 kDa


    Exposure time: 3 minutes


    This data was developed using ab124764, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (Unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with IFN-alpha
    Lane 3 : K562 cell lysate
    Lane 4 : U937 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/200 dilution

    Predicted band size: 64 kDa



    This data was developed using ab124764, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    OI-RD Scanning - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    This data was developed using ab124764, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
    Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)

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