Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸ 10 µl 137 366 ₸

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Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Interferon alpha/beta receptor 1 antibody [EPR6244]
See all Interferon alpha/beta receptor 1 primary antibodies
Description

Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1
Host species

Rabbit
Specificity

In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 100-200. The exact sequence is proprietary.
Database link: P17181
Positive control
- HeLa, HeLa treated with IFN-alpha, K562 and U937 cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.40 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6244
Isotype

IgG
Research areas

Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution

Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : Interferon alpha/beta receptor 1 knockout HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 64 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab124764 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab124764 was shown to specifically react with Interferon Receptor alpha in wild-type HeLa cells as signal was lost in Interferon alpha/beta receptor 1 knockout cells. Wild-type and Interferon alpha/beta receptor 1 knockout samples were subjected to SDS-PAGE. Ab124764 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 (ab124764) at 1/1000 dilution

Predicted band size: 64 kDa
Observed band size: 90 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST

In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

Lane 1 : HEK293 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : LNCaP whole cell lysate
Lane 4 : SH-SY5Y whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 90-130 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (unpurified)

Lane 1 : Mouse brain whole cell lysate
Lane 2 : Mouse heart whole cell lysate
Lane 3 : Mouse kidney whole cell lysate
Lane 4 : Mouse spleen whole cell lysate
Lane 5 : Rat brain whole cell lysate
Lane 6 : Rat heart whole cell lysate
Lane 7 : Rat kidney whole cell lysate
Lane 8 : Rat spleen whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated. at 1/2000 dilution

Predicted band size: 64 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST.

In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (Unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with IFN-alpha
Lane 3 : K562 cell lysate
Lane 4 : U937 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/200 dilution

Predicted band size: 64 kDa
OI-RD Scanning - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

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