Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Interferon alpha/beta receptor 1 antibody [EPR6244]
    See all Interferon alpha/beta receptor 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1

  • Host species

    Rabbit

  • Specificity

    In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 100-200. The exact sequence is proprietary.
    Database link: P17181

  • Positive control

    • HeLa, HeLa treated with IFN-alpha, K562 and U937 cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : Interferon alpha/beta receptor 1 knockout HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 64 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab124764 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab124764 was shown to specifically react with Interferon Receptor alpha in wild-type HeLa cells as signal was lost in Interferon alpha/beta receptor 1 knockout cells. Wild-type and Interferon alpha/beta receptor 1 knockout samples were subjected to SDS-PAGE. Ab124764 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 (ab124764) at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : LNCaP whole cell lysate
    Lane 4 : SH-SY5Y whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 64 kDa
    Observed band size: 90-130 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse brain whole cell lysate
    Lane 2 : Mouse heart whole cell lysate
    Lane 3 : Mouse kidney whole cell lysate
    Lane 4 : Mouse spleen whole cell lysate
    Lane 5 : Rat brain whole cell lysate
    Lane 6 : Rat heart whole cell lysate
    Lane 7 : Rat kidney whole cell lysate
    Lane 8 : Rat spleen whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated. at 1/2000 dilution

    Predicted band size: 64 kDa


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST.

    In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (Unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with IFN-alpha
    Lane 3 : K562 cell lysate
    Lane 4 : U937 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/200 dilution

    Predicted band size: 64 kDa

  • OI-RD Scanning - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    OI-RD Scanning - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
    Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)

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