Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1
  • Suitable for: WB, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Interferon alpha/beta receptor 1 antibody [EP899Y]
    See all Interferon alpha/beta receptor 1 primary antibodies

  • Description

    Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for the 90kda form and the full-length form of Interferon alpha/beta receptor 1 protein.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 500-600 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and K562 cell lysates. Flow cyt: K562 cells. IP: K-562 cell lysate

  • General notes

    We recommend ab124764 as a better alternative to ab45172.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
    All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/1000 dilution

    Lane 1 : Wild-type (HeLa) whole cell lysate
    Lane 2 : IFNAR1 (Interferon Receptor alpha) knockout (HeLa) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 64 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab45172 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab45172 was shown to recognize Interferon alpha/beta receptor 1 in wild-type WT (HeLa) cells as signal was lost at the expected MW in IFNAR1 (Interferon Receptor alpha) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IFNAR1 (Interferon Receptor alpha) knockout samples were subjected to SDS-PAGE. Ab45172 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
    Immunoprecipitation - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

    Purified ab45172 at 1/50 dilution (2µg) immunoprecipitating Interferon alpha/beta receptor 1 in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab45172 + K-562 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45172 in K-562 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 130 kDa

  • Flow Cytometry - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
    Flow Cytometry - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
    Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green).
  • Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
    Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
    Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg

    Secondary
    Goat anti rabbit HRP labelled. at 1/2000 dilution

    Predicted band size: 64 kDa
    Observed band size: 130,90 kDa
    why is the actual band size different from the predicted?

  • Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
    Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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