Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
![Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-324331-anti-interferon-alpha-beta-receptor-1-antibody-ep899y-western-blot.jpg "Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1
- Suitable for: WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Interferon alpha/beta receptor 1 antibody [EP899Y]
See all Interferon alpha/beta receptor 1 primary antibodies -
Description
Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1 -
Host species
Rabbit -
Specificity
This antibody is specific for the 90kda form and the full-length form of Interferon alpha/beta receptor 1 protein. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human
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Immunogen
Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 500-600 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa and K562 cell lysates. Flow cyt: K562 cells. IP: K-562 cell lysate
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General notes
We recommend ab124764 as a better alternative to ab45172.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP899Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/1000 dilution
Lane 1 : Wild-type (HeLa) whole cell lysate
Lane 2 : IFNAR1 (Interferon Receptor alpha) knockout (HeLa) whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 64 kDaLanes 1 - 2: Merged signal (red and green). Green - ab45172 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab45172 was shown to recognize Interferon alpha/beta receptor 1 in wild-type WT (HeLa) cells as signal was lost at the expected MW in IFNAR1 (Interferon Receptor alpha) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IFNAR1 (Interferon Receptor alpha) knockout samples were subjected to SDS-PAGE. Ab45172 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-7-anti-interferon-alpha-beta-receptor-1-antibody-ep899y-immunoprecipitation-k562-human.jpg)
Immunoprecipitation - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)Purified ab45172 at 1/50 dilution (2µg) immunoprecipitating Interferon alpha/beta receptor 1 in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab45172 + K-562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45172 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 130 kDa -
![Flow Cytometry - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-57337-anti-interferon-alpha-beta-receptor-1-antibody-ep899y-flow-cytometry.jpg)
Flow Cytometry - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green). -
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-57338-anti-interferon-alpha-beta-receptor-1-antibody-ep899y-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg
Secondary
Goat anti rabbit HRP labelled. at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 130,90 kDa why is the actual band size different from the predicted?
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![Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-6-benefits-of-recombinant-antibodies.png)
Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
