Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸ 10 µl 137 366 ₸

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Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1
Suitable for: WB, Flow Cyt, IP
Knockout validated
Reacts with: Human

Product name

Anti-Interferon alpha/beta receptor 1 antibody [EP899Y]
See all Interferon alpha/beta receptor 1 primary antibodies
Description

Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1
Host species

Rabbit
Specificity

This antibody is specific for the 90kda form and the full-length form of Interferon alpha/beta receptor 1 protein.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 500-600 (C terminal). The exact sequence is proprietary.
Positive control
- WB: HeLa and K562 cell lysates. Flow cyt: K562 cells. IP: K-562 cell lysate
General notes
We recommend ab124764 as a better alternative to ab45172.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP899Y
Isotype

IgG
Research areas

Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/1000 dilution

Lane 1 : Wild-type (HeLa) whole cell lysate
Lane 2 : IFNAR1 (Interferon Receptor alpha) knockout (HeLa) whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 64 kDa

Lanes 1 - 2: Merged signal (red and green). Green - ab45172 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab45172 was shown to recognize Interferon alpha/beta receptor 1 in wild-type WT (HeLa) cells as signal was lost at the expected MW in IFNAR1 (Interferon Receptor alpha) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IFNAR1 (Interferon Receptor alpha) knockout samples were subjected to SDS-PAGE. Ab45172 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

Purified ab45172 at 1/50 dilution (2µg) immunoprecipitating Interferon alpha/beta receptor 1 in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab45172 + K-562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45172 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 130 kDa
Flow Cytometry - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green).
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg

Secondary
Goat anti rabbit HRP labelled. at 1/2000 dilution

Predicted band size: 64 kDa
Observed band size: 130,90 kDa
why is the actual band size different from the predicted?
Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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