Anti-IRF3 antibody [IRF35I218] (ab50772)
![Anti-IRF3 antibody [IRF35I218] (ab50772)](https://www.abcam.com/ps/products/50/ab50772/Images/ab50772-62462-anti-irf3-antibody-irf35i218-western-blot.jpg "Anti-IRF3 antibody [IRF35I218] (ab50772)")
Key features and details
- Mouse monoclonal [IRF35I218] to IRF3
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-IRF3 antibody [IRF35I218]
See all IRF3 primary antibodies -
Description
Mouse monoclonal [IRF35I218] to IRF3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Recombinant fragment: VAWVNKSRTR FRIPWKHGLR QDAQQEDFGI FQAWAEATGA YVPGRDKPDL PTWKRNFRSA LNRKEGLRLA EDRSKD, corresponding to amino acids 24-150 of Human IRF3
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Positive control
- HEK293 whole cell lysate RIPA lysate of HeLa cells Hela cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.316% Tris HCl, 1% BSA, 1.16% Sodium chloride -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Concentrated and dialyzed culture medium (protein free medium) of hybridoma -
Clonality
Monoclonal -
Clone number
IRF35I218 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg
Secondary
Anti mouse IgG at 1/2500 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (ab50772)](https://www.abcam.com/ps/products/50/ab50772/Images/ab50772-210362-anti-irf3-antibody-irf35i218-flow-cytometry.jpg)
Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (ab50772)Overlay histogram showing HeLa cells stained with ab50772 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50772, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (ab50772)](https://www.abcam.com/ps/products/50/ab50772/Images/ab50772-62463-anti-irf3-antibody-irf35i218-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (ab50772)Immunofluorescent staining of HeLa cells with a 1/10 dilution of ab50772 as primary antibody and AlexaFluor®488 conjugated Goat Anti-mouse IgG diluted 1/200 as the secondary antibody -
![Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)](https://www.abcam.com/ps/products/50/ab50772/Images/ab50772-62461-anti-irf3-antibody-irf35i218-western-blot.jpg)
Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg
Secondary
Anti-mouse IgG at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg Secondary Anti-mouse IgG at 1/1000 dilution
