Antibodies, Proteins, Kits and Reagents for Life Science

334 369 ₸ Product size

100 µl 334 369 ₸

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HRP Anti-IRF3 antibody [EPR2418Y] (ab205443)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR2418Y] to IRF3
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-IRF3 antibody [EPR2418Y]
See all IRF3 primary antibodies
Description

HRP Rabbit monoclonal [EPR2418Y] to IRF3
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human IRF3 aa 50-150. The exact sequence is proprietary.
Database link: Q14653
Positive control
- WB: HeLa, Jurkat and wild-type HAP1 whole cell lysates. IHC-P: Normal human tonsil tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2418Y
Isotype

IgG
Research areas

Western blot - HRP Anti-IRF3 antibody [EPR2418Y] (ab205443)

All lanes : HRP Anti-IRF3 antibody [EPR2418Y] (ab205443) at 1/2000 dilution

Lane 1 : HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3 : Wild-type HAP1 cell lysate at 20 µg
Lane 4 : IRF3 knockout HAP1 cell lysate at 20 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 47 kDa
Observed band size: 51 kDa
why is the actual band size different from the predicted?

Exposure time: 20 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205443 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-IRF3 antibody [EPR2418Y] (ab205443)

IHC image of IRF3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil tissue*, performed on a Leica BOND^TM. The section was pre-treated using heat mediated antigen retrieval with TRIS/EDTA (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab205443, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
HRP Anti-IRF3 antibody [EPR2418Y] (ab205443)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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