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Cancer Tumor immunology Cytokines Interferons

Anti-IRF3 antibody (ab245341)

Anti-IRF3 antibody (ab245341)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to IRF3
  • Suitable for: WB, IP
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-IRF3 antibody
    See all IRF3 primary antibodies
  • Description

    Rabbit polyclonal to IRF3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide within Mouse IRF3 aa 375-419. The exact sequence is proprietary. NP_058545.1
    Database link: P70671

  • Positive control

    • WB: Mouse bone marrow dendritic whole cell lysate; NIH/3T3 whole cell lysate. IP: Mouse bone marrow dendritic whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245341 was affinity purified using an epitope specific to IRF3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Immunology
    • Innate Immunity
    • TLR Signaling

Images

  • Western blot - Anti-IRF3 antibody (ab245341)
    Western blot - Anti-IRF3 antibody (ab245341)
    All lanes : Anti-IRF3 antibody (ab245341) at 0.4 µg/ml

    Lane 1 : Mouse bone marrow dendritic whole cell lysate at 50 µg
    Lane 2 : Mouse bone marrow dendritic whole cell lysate at 15 µg
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Predicted band size: 47 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-IRF3 antibody (ab245341)
    Immunoprecipitation - Anti-IRF3 antibody (ab245341)

    IRF was immunoprecipitated from mouse bone marrow dendritic whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
    ab245341 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245341 IP in mouse bone marrow dendritic whole cell lysate.
    Lane 2: Control IgG IP in mouse bone marrow dendritic whole cell lysate.

    Chemiluminescence detection: 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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