Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Cancer Tumor immunology Cytokines Interferons

Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)

Price and availability

345 091 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2346] to IRF3 (phospho S386)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

You may also be interested in

Product image
Human IRF2 knockout A549 cell pellet (ab279241)
Product image
Anti-IRF3 antibody (ab245340)
Product image
Anti-Interferon alpha 1 antibody - BSA and Azide free (Capture) (ab259641)
Product image
Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)

Overview

  • Product name

    Anti-IRF3 (phospho S386) antibody [EPR2346]
    See all IRF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR2346] to IRF3 (phospho S386)
  • Host species

    Rabbit
  • Specificity

    IRF3 (phospho S386) antibody (ab76493) detects IRF3 when phosphorylated on Serine 386.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human IRF3 (phospho S386).
    Database link: Q14653

  • Positive control

    • WB: MCF7 cells treated with Calyculin A.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2346
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Immunology
    • Innate Immunity
    • TLR Signaling

Images

  • Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)
    Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)
    All lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) at 0.2 µg/ml (purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) treated with calyculin A for 45 minutes whole cell lysates
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) treated with calyculin A for 45 minutes whole cell lysates. Then the membrane was incubated with phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

    We are unsure to define the extra band at 37KD.

  • Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)
    Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)
    All lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) at 1/20000 dilution (Unpurified)

    Lane 1 : MCF7 cell lysate - treated with Calyculin A
    Lane 2 : MCF7 cell lysate – untreated

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 47 kDa



    Blocking buffer - 5% NFDM/TBST

    Diluting buffer - 1% BSA

  • Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)
    Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)

    Dot blot analysis of IRF3 single phospho peptide pS386 (lane 1) and IRF3 non-phospho peptide (lane 2) with unpurified ab76493 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.

  • Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)
    Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)

  •  
  • Product image

    Anti-IRF3 antibody [EP2419Y] (ab76409)

    Applications: Flow Cyt, IHC-P, IP, WB

  •  
  • Product image

    Anti-IRF3 (phospho S396) antibody (ab138449)

    Applications: WB

  •  
  • Product image

    Anti-IRF3 antibody (ab218160)

    Applications: IHC-P

  •  
  • Product image

    Anti-IRF3 antibody [EPR2418Y] (ab68481)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859)

    Applications: Dot, WB

  •  
  • Product image

    Anti-IRF3 (phospho S386) antibody (ab192796)

    Applications: WB

  •  
  • Product image

    Anti-IRF3 antibody [EP2419Y] - BSA and Azide free (ab201810)

    Applications: Flow Cyt, IHC-P, IP, WB

  •  
  • Product image

    Anti-IRF3 antibody (ab238521)

    Applications: ICC/IF, WB

  •  
  • Product image

    HRP Anti-IRF3 antibody [EPR2418Y] (ab205443)

    Applications: IHC-P, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.