Anti-IRF3 antibody (ab245340)
Key features and details
- Rabbit polyclonal to IRF3
- Suitable for: WB, IP
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-IRF3 antibody
See all IRF3 primary antibodies -
Description
Rabbit polyclonal to IRF3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
-
Immunogen
Synthetic peptide within Mouse IRF3 aa 275-325. The exact sequence is proprietary. NP_058545.1
Database link: P70671 -
Positive control
- WB: Mouse bone marrow dendritic cell lysate; NIH/3T3 whole cell lysate. IP: Mouse bone marrow dendritic cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245340 was affinity purified using an epitope specific to IRF3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-IRF3 antibody (ab245340)All lanes : Anti-IRF3 antibody (ab245340) at 0.4 µg/ml
Lane 1 : Mouse bone marrow dendritic cell lysate at 50 µg
Lane 2 : Mouse bone marrow dendritic cell lysate at 15 µg
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-IRF3 antibody (ab245340)IRF3 was immunoprecipitated from mouse bone marrow dendritic cell lysate (1 mg for IP, 20% of IP loaded) with ab245340 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245340 at 1 µg/ml.
Lane 1: ab245340 IP in mouse bone marrow dendritic cell lysate.
Lane 2: Control IgG IP in mouse bone marrow dendritic cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
