Anti-CHMP7 antibody (ab242221)
Key features and details
- Rabbit polyclonal to CHMP7
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CHMP7 antibody
See all CHMP7 primary antibodies -
Description
Rabbit polyclonal to CHMP7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human CHMP7 aa 300-350. The exact sequence is proprietary.
Database link: Q8WUX9 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: CHMP7 IP in HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to CHMP7 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CHMP7 antibody (ab242221)All lanes : Anti-CHMP7 antibody (ab242221) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 3 minutesLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-CHMP7 antibody (ab242221)CHMP7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242221 at 20 µl/reaction.
Lysates prepared in NETN buffer.
All lanes : Anti-CHMP7 antibody (ab242221) at 1/250 dilution
Lane 1 : ab242221 IP in HEK-293T whole cell lysate
Lane 2 : Control IgG IP in HEK-293T whole cell lysate
Developed using the ECL technique.
Exposure time: 30 seconds
