Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µg 321 638 ₸ 10 µg 107 212 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [14E5] to Sez6
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat

Product name

Anti-Sez6 antibody [14E5]
See all Sez6 primary antibodies
Description

Rat monoclonal [14E5] to Sez6
Host species

Rat
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

WB
Mouse

Rat

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat brain lysate. IHC-P: Mouse and rat cerebrum and hippocampus tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Mouse Rat
WB	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

14E5
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-Sez6 antibody [14E5] (ab252863)

All lanes : Anti-Sez6 antibody [14E5] (ab252863) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution

Predicted band size: 107 kDa
Observed band size: 170 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed was in consistent with the literature (PMID: 27716410).

Exposure time: 180 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Sez6 with ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the rat hippocampus. The section was incubated with ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Sez6 with ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the rat cerebrum. The section was incubated with ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Sez6 with ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the mouse hippocampus. The section was incubated with ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Sez6 with ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the mouse cerebrum. The section was incubated with ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Sez6 antibody [14E5] (ab252863)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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