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Human E2F4 knockout HEK293T cell pellet (ab279031)

Overview

  • Product name

    Human E2F4 knockout HEK293T cell pellet
    See all E2F4 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2 and Insertion of the selection cassette in exon2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.

  • Tissue specificity

    Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

  • Sequence similarities

    Belongs to the E2F/DP family.

  • Developmental stage

    Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.

  • Post-translational
    modifications

    Differentially phosphorylated in vivo.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Alternative names

    • E2F 4
    • E2F transcription factor 4
    • E2F transcription factor 4 p107/p130 binding
    • E2F-4
    • E2F4
    • E2F4_HUMAN
    • p107/p130 binding protein
    • Transcription factor E2F 4
    • Transcription factor E2F4
    see all

Properties

Images

  • Western blot - Human E2F4 knockout HEK293T cell pellet (ab279031)
    Western blot - Human E2F4 knockout HEK293T cell pellet (ab279031)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:E2F4 knockout HEK293T cell lysate (20 ug)
    Lane 3:K-562 cell lysate (20 ug)
    Lane 4:Jurkat cell lysate (20 ug)

    ab150360 was shown to specifically react with E2F4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266119 (knockout cell lysate ab257932) was used. Wild-type and E2F4 knockout samples were subjected to SDS-PAGE. ab150360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human E2F4 knockout HEK293T cell pellet (ab279031)
    Sanger Sequencing - Human E2F4 knockout HEK293T cell pellet (ab279031)

    Allele-1: 4 bp deletion in exon2

     

  • Sanger Sequencing - Human E2F4 knockout HEK293T cell pellet (ab279031)
    Sanger Sequencing - Human E2F4 knockout HEK293T cell pellet (ab279031)

    Allele-2: Insertion of the selection cassette in exon2

     

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