Anti-Cardiotin antibody [R2G] (ab8962)
![Anti-Cardiotin antibody [R2G] (ab8962)](https://www.abcam.com/ps/products/8/ab8962/Images/ab8962-395635-AntiCardiotin-antibody-R2G-ab8962.png "Anti-Cardiotin antibody [R2G] (ab8962)")
Key features and details
- Mouse monoclonal [R2G] to Cardiotin
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig, Zebrafish
- Isotype: IgM
Overview
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Product name
Anti-Cardiotin antibody [R2G] -
Description
Mouse monoclonal [R2G] to Cardiotin -
Host species
Mouse -
Specificity
This antibody reacts with cardiotin, a mitochondrion-associated protein which is present in cardiomyocytes and skeletal muscle. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig, Zebrafish -
Immunogen
Total protein extract of chicken gizzard
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General notes
Mitochondrial membrane proteins have been shown to be involved in several cellular processes such as the regulation of apoptosis and the energy metabolism. Cardiotin antibodies, which are reactive with mitochondrial proteins, have proven to be early indicators of ischemia induced changes in the myocardium.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
R2G -
Myeloma
Sp2/0-Ag14 -
Isotype
IgM -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
Indirect immunofluorescence analysis of swine heart cells labelling cardiotin with ab8962.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)](https://www.abcam.com/ps/products/8/ab8962/Images/ab8962-395634-AntiCardiotin-antibody-R2G-ab8962.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)Indirect immunofluorescence analysis of swine heart cells labelling cardiotin with ab8962.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)](https://www.abcam.com/ps/products/8/ab8962/Images/ab8962_1.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)Human atrial cardiomyocytes stained with the monoclonal cardiotin antibody R2G.
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![Western blot - Anti-Cardiotin antibody [R2G] (ab8962)](https://www.abcam.com/ps/products/8/ab8962/Images/ab8962_2.gif)
Western blot - Anti-Cardiotin antibody [R2G] (ab8962)Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G.
Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)](https://www.abcam.com/ps/products/8/ab8962/Images/Cardiotin-Primary-antibodies-ab8962-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)Immunofluorescence staining of a 7 days old zebrafish embryo