All lanes : Anti-Actin [IGX3831R-3] at 1 µg/ml

Lane 1 : Human colon tissue lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lane 6 : PANC1 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lane 8 : Human skeletal muscle tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 42 kDa


Exposure time: 2 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes.  The membrane was then blocked for an hour using 3% milk before being incubated with ab218787 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

Ab218787 staining Actin in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab218787 at a 5µg/ml concentration, then detected with a goat anti-human (Alexa Fluor^® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

 

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Ab218787 staining Actin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab218787 at a 5µg/ml concentration, then detected with a goat anti-human (Alexa Fluor^® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

 

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).