Anti-PML Protein (phospho S518) antibody [EPR6151] (ab128932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6151] to PML Protein (phospho S518)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-PML Protein (phospho S518) antibody [EPR6151]
See all PML Protein primary antibodies -
Description
Rabbit monoclonal [EPR6151] to PML Protein (phospho S518) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
A phospho specific peptide corresponding to residues surrounding Serine 518 of Human PML Protein.
-
Positive control
- HeLa lysate, Human astrocytoma or breast carcinoma tissue.
-
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6151 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-PML Protein (phospho S518) antibody [EPR6151] (ab128932) at 1/1000 dilution
Lane 1 : HeLa lysate (untreated)
Lane 2 : HeLa lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat
anti-rabbit at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
-
ab128932, at a 1/100 dilution, staining Human PML Protein in astrocytoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
ab128932, at a 1/50 dilution, staining Human PML Protein in breast carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-