Anti-Phospho Serine/Threonine Chk1/Chk2 substrate antibody (ab131023)
Key features and details
- Rabbit polyclonal to Phospho Serine/Threonine Chk1/Chk2 substrate
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospho Serine/Threonine Chk1/Chk2 substrate antibody -
Description
Rabbit polyclonal to Phospho Serine/Threonine Chk1/Chk2 substrate -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Phospho Serine/Threonine Chk1/Chk2 substrate aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P04637 -
Positive control
- This antibody gave a positive signal in HeLa and HeLa UV irradiated whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).Target
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Relevance
Several protein kinases mediate cellular responses to DNA damage. This includes the serine/threonine kinases Chk1 and Chk2. In response to DNA damage Chk1 and Chk2 phosphorylate a large range of proteins on Serine (S) or Threonine (T) residues on RxxS/T degenerated consensus sites. -
Alternative names
- Phospho Chk1 substrate antibody
- Phospho Chk1/Chk2 substrate antibody
- Phospho Chk2 substrate antibody
see all
Images
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Western blot - Anti-Phospho Serine/Threonine Chk1/Chk2 substrate antibody (ab131023)All lanes : Anti-Phospho Serine/Threonine Chk1/Chk2 substrate antibody (ab131023) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - UV Irradiated
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 4 : Hela Whole Cell Lysate - UV Irradiated with Immunising peptide at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate withAnti-HIF-1 alpha antibody [H1alpha67] (ab1)
Lane 6 : Hela Whole Cell Lysate - UV Irradiated withAnti-HIF-1 alpha antibody [H1alpha67] (ab1)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab131023 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (1)
ab131023 has been referenced in 1 publication.
- Renga B et al. Cystathionine ?-lyase, a H2S-generating enzyme, is a GPBAR1-regulated gene and contributes to vasodilation caused by secondary bile acids. Am J Physiol Heart Circ Physiol 309:H114-26 (2015). PubMed: 25934094
Images
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Western blot - Anti-Phospho Serine/Threonine Chk1/Chk2 substrate antibody (ab131023)All lanes : Anti-Phospho Serine/Threonine Chk1/Chk2 substrate antibody (ab131023) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - UV Irradiated
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 4 : Hela Whole Cell Lysate - UV Irradiated with Immunising peptide at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate withAnti-HIF-1 alpha antibody [H1alpha67] (ab1)
Lane 6 : Hela Whole Cell Lysate - UV Irradiated withAnti-HIF-1 alpha antibody [H1alpha67] (ab1)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab131023 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.