Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17862-7] to gamma Sarcoglycan - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free
    See all gamma Sarcoglycan primary antibodies

  • Description

    Rabbit monoclonal [EPR17862-7] to gamma Sarcoglycan - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251394 is the carrier-free version of ab203113. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251394 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)

    This data was developed using ab203113, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weak cytoplasmic staining observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/10000 dilution + Human skeletal muscle whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 15 seconds


    This data was developed using ab203113, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This protein is expressed in skeletal and heart muscle.

  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/1000 dilution + Mouse muscle lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 30 seconds


    This data was developed using ab203113, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution + Mouse heart tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 10 seconds


    This data was developed using ab203113, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution + MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 3 minutes


    This data was developed using ab203113, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    All lanes : Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution

    Lane 1 : Human fetal brain whole tissue lysate
    Lane 2 : Human fetal heart whole tissue lysate
    Lane 3 : Human fetal kidney whole tissue lysate
    Lane 4 : Human fetal spleen whole tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 15 seconds


    This data was developed using ab203113, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This protein is expressed in skeletal and heart muscle.

    ab181602 Anti-GAPDH antibody [EPR16891] control used.

  • Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    This data was developed using ab203113, the same antibody clone in a different buffer formulation.Human gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203113 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203113 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
    Lane 1: Human fetal kidney whole cell lysate 10 µg (Input). Lane 2: ab203113 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203113 in Human fetal kidney whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 Seconds Blocking/Dilution buffer 5% NFDM/TBST.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    This data was developed using ab203113, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. No staining is observed on Human colon tissue. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)
    Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (ab251394)

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