Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22492-2] to KIR2DL1 + KIR2DL2 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR22492-2] to KIR2DL1 + KIR2DL2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, IHC-Pmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human KIR2DL1 recombinant protein (aa1-245); His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221). IHC-P: Human endometrium tissue. ICC/IF: NK-92 cells (treated with 5-aza-2-deoxycytidine(2µM) for 48h). Flow Cyt: Human PBMCs. IP: NK-92 (treated with 2µM 5-aza-2-deoxycytidine for 48h) whole cell lysate.

  • General notes

    Ab255806 is the carrier-free version of ab224696. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255806 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)
    Immunoprecipitation - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)

    KIR2DL1 + KIR2DL2 were immunoprecipitated from 0.35 mg of NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) (treated with 2μM 5-aza-2-deoxycytidine for 48h) whole cell lysate with ab224696 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224696 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: NK-92 (treated as above) whole cell lysate 10 μg (Input).
    Lane 2: ab224696 IP in NK-92 (treated as above) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224696 in NK-92 (treated as above) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 100 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252936).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224696).

  • Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)
    Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells (+/- treatment with 5-aza-2-deoxycytidine(2µM) for 48h) labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in NK-92 cells treated with 5-aza-2-deoxycytidine(2µM) for 48h. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224696).

  • Flow Cytometry - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)
    Flow Cytometry - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)

    Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/60 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab224696 (Right). Then stained with anti-CD56 conjugated to BV421.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224696).

  • Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)
    Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] - BSA and Azide free (ab255806)

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